| 000 | 02315nam a2200277Ia 4500 | ||
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| 003 | MX-MdCICY | ||
| 005 | 20260521091655.0 | ||
| 040 | _cCICY | ||
| 090 | _aB-19237 | ||
| 245 | 1 | 0 | _apH-controlled reversible deep-eutectic solvent based enzyme system for simultaneous extraction and in-situ separation of isoflavones from Pueraria lobata |
| 490 | 0 | _vSeparation and Purification Technology, 292, p.120992, 2022 | |
| 520 | 3 | _aIn this study, we developed an efficient and green pH-controlled reversible deep-eutectic solvents (DESs)based enzyme system for efficient extraction, transformation and in-situ separation of natural products from herbs. As a case study, seven isoflavones in the root of Pueraria lobata were selected as target compounds. A series of DESs were firstly prepared and the extraction efficiency for target compounds with or without cellulase catalysis was investigated. We found that [N4444][Cl]:ethylene glycol (1:2)with cellulase addition show the highest extraction efficiency for isoflavones. More importantly, the phase behavior of [N4444][Cl]:ethylene glycol (1:2)based cellulase system was found to be pH-controlled. When pH is 5.0, the system is a homogeneous single-phase system, which is conducive to the extraction of natural products. When pH is 6.4, the system changes from the single-phase to the heterogeneous two-phase, to realize the in-situ separation of the compounds. Therefore, by adjusting the pH of the system, the in-situ separation of isoflavones was successfully realized with high recovery yields. The pH-controlled DESs based enzyme-assisted extraction system overcomes the incompatibility between enzyme catalysis and conventional organic solvent in extraction, which could efficiently extract seven isoflavones from Pueraria lobata root. | |
| 650 | 1 | 4 | _aDEEP-EUTECTIC SOLVENTS |
| 650 | 1 | 4 | _aCELLULASE |
| 650 | 1 | 4 | _aULTRASOUND |
| 650 | 1 | 4 | _aPH-CONTROLLED SOLVENT SYSTEM |
| 650 | 1 | 4 | _aPUERARIA LOBATA |
| 700 | 1 | 2 | _aWang, T., |
| 700 | 1 | 2 | _aGuo, Q. |
| 700 | 1 | 2 | _aYang, H. |
| 700 | 1 | 2 | _aGao, W. |
| 700 | 1 | 2 | _aLi, P. |
| 856 | 4 | 0 |
_uhttps://drive.google.com/file/d/1YCrSD-jJcPvTpayUJtbYyHmmeUOu0jUX/view?usp=drivesdk _zPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx |
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