Transgenic broccoli with high levels of Bacillus thuringiensis Cry1C protein control diamondback moth larvae resistant to Cry1A or Cry1C.
Material type:
TextSeries: ; Molecular Breeding, 5(2), p.131-141, 1999Contained works: - Cao, J
- Tang, J.D
- Strizhov, N
- Shelton, A.M
- Earle, E.D
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A synthetic Bacillus thuringiensis (Bt)cry1C gene was introduced into broccoli (Brassica oleracea ssp. italica)by Agrobacterium-mediated transformation. Twenty-one Cry1C transgenic plants were regenerated from 400 hypocotyl and petiole explants. Variable amounts of stable steady- state cry1C mRNA accumulated in different transgenic plants. Cry1C protein (up to 0.4
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