An improved HPLC assay for phosphatidylcholine hydroperoxides (PCOOH)in human plasma with synthetic PCOOH as internal standard
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TextSeries: ; Journal of Chromatography B, 857(1), p.158-163, 2007Contained works: - Hui, S
- Chiba, H
- Sakurai, T
- Asakawa, C
- Nagasaka, H
- Murai, T
- Ide, H
- Ide, H
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Quantitative and qualitative analyses of 1-palmitoyl-2-linoleoyl-phosphatidylcholine monohydroperoxide [PC 16:0/18:2-OOH]and 1-stearoyl-2-linoleoyl-phosphatidylcholine monohydroperoxide [PC 18:0/18:2-OOH]in human plasma were improved by chemiluminescence HPLC usingsynthetic 1-stearoyl-2-erucoyl-phosphatidylcholine monohydroperoxide (PC 18:0/22:1-OOH)as internal standard. The calibration curves of synthetic PC 16:0/18:2-OOH and PC 18:0/18:2-OOH, obtained by their direct injections with the IS into the HPLC system, were linear throughout the calibration range (10-1000 pmol).Within-day and between-day coefficients of variation were below
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