02823nam a2200277Ia 4500003001000000005001700010040000900027245013500036490005000171500001500221520190100236650002402137650001402161650001402175650001702189650003702206700001702243700001302260700001402273700002002287700001302307700001202320700001402332856015802346008004102504MX-MdCICY20260521092148.0  cCICY10aEpigenetic Evaluation of Melatonin Application in Bean (Phaseolus vulgaris L.) Genotypes Under Drought and Salt Stress Conditions.0 aPlant Molecular Biology Reporter, 1-19, 2025.  aArtículo3 aPhaseolus vulgaris L. is an important legume used in human nutrition because it contains high protein, low fat, and high levels of vitamins and minerals. Plants are exposed to various biotic and abiotic stresses throughout their lives and have developed various resistance mechanisms. Melatonin, an animal hormone, plays a vital role in processing various metabolic processes and plant stress defense mechanisms in plants. It also supports plant growth and photosynthetic activity and regulates circadian rhythms, flowering, stress resistance, and phytohormone-mediated signal transduction pathways. This study aimed to determine the effect of melatonin applied exogenously to Serra and Elkoca 05 genotypes under drought and salt stress on biochemical and epigenetic changes in root and leaf tissues. Seeds germinated for 7 days and were then grown in Hoagland solution containers until the 5-leaf stage. Melatonin was applied to foliar by spraying at 0 and 200 µM doses. After 24 h, they were placed in Hoagland solution containing different doses of NaCl (0 and 150 mM) and PEG6000 (0 and 20%). In the research, biochemical MDA and H2O2, antioxidant enzyme activity (SOD, POD, and CAT), and DNA methylation changes (CRED-iPBS) were detected. As a result of the research, the stresses applied on both genotypes caused an increase in the amounts of MDA and H2O2; it was determined that melatonin applied during salt stress caused a decrease in these parameters. SOD, POD, and CAT antioxidant activities of genotypes varied according to applications. On the other hand, according to DNA methylation results, stress conditions and melatonin application caused changes in DNA methylation and polymorphism rates. In summary, our research indicates that the application of melatonin proves to be a successful approach for enhancing the resilience of bean plants against both salt and drought stress.14aSALT-DROUGHT STRESS14aMELATONIN14aCRED-IPBS14aPOLYMORPHISM14aCOMMON BEAN (PHASEOLUS VULGARIS)12aAydinyurt, R12aYagci, S12aYaprak, E12aKasapoglu, A. G12aMuslu, S12aUcar, S12aAydin, M.40uhttps://drive.google.com/file/d/19IqULZbMqFCH_wNxVtK5M49aqFvpYd83/view?usp=drive_linkzPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx251009s9999    xx            000 0 und d