02455nam a2200229Ia 4500 MX-MdCICY 20260521092145.0 CICY B-21839 Isolation and Culture of Pineapple (Ananas comnosus) Protoplast Catrina: The International Journal of Environmental Sciences, 2(1): p.97-103, 2007 Artículo In vitro full expended healthy leaves and sterilized in vivo leaves of pineapple (Ananas cv. smooth cayenne) plants were taken and prepared under aseptic conditions as different sources of explants. Also, different enzymes mixtures, incubation periods, osmotic pressure factors, shaking periods and speeds were concerned in combination with explants sources during protoplast isolation stage. In addition, sieve size and centerfugation speed were evaluated in combination with explants source during purification stage. Moreover, medium type protoplast density, auxin/cytokinin concentration ratio, and antibiotic were tested in combination with explants source during protoplast culturing. It is found that in vitro and sterilized in vivo explants source succeeded in maximizing protoplast yield. Also, using of enzymes mixture consists of 1.0percent cellulase + 0.5percent macerozyme was superior in increasing protoplast yield Moreover, using of sucrose at rate of 13.6g /100ml as osmotic pressure factor and incubation for 20 hours then, shaking for 15 min with speed rate 75 rpm succeeded in enhancing the highest protoplast isolation of pineapple. Meanwhile, using of 25 µM pore size mesh sieve and centrifugation at the rate of 1000rpm maximized protoplast purification. Moreover, culturing of protoplast KAO and Michayluk medium supplemented with 3.0 mg/l NAA and 0.2 mg/l BAP as well as the combination of antibiotic (0.4 mg/l Ampicilin + 0.1 g/l gentamycin + 0.1 g/l tetracycline) and using protoplast density at the rate of 2.5 x 104 induced the best protoplast viability and development of pineapple explants. PINEAPPLE TISSUE CULTURE CELL DIVISION PROTOPLAST ISOLATION Hagagy, N.;Mougheith, M.;Saleh, M.;Gendiah, H.;Mohamed, S.;Hassan, S. https://drive.google.com/file/d/12Piy0S9pXrehbFBqxzBs9f75ULLyF6bO/view?usp=drive_link Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 251009s9999 xx 000 0 und d 37859 37859 0 0 Loc 0 0 F1 CICY CICY RE 2025-10-09 0 B-21839 2025-10-09 16:07:07 2025-10-09 REF1