01553nam a2200145Ia 4500003001000000005001700010040000900027245007600036490003800112500001500150520096800165700007501133856015801208008004101366MX-MdCICY20260521092145.0  cCICY10aEngineering synthetic phosphorylation signaling networks in human cells0 aScience, 387(6729), p.74-81, 2025  aArtículo3 aProtein phosphorylation signaling networks have a central role in how cells sense and respond to their environment. We engineered artificial phosphorylation networks in which reversible enzymatic phosphorylation cycles were assembled from modular protein domain parts and wired together to create synthetic phosphorylation circuits in human cells. Our design scheme enabled model-guided tuning of circuit function and the ability to make diverse network connections; synthetic phosphorylation circuits can be coupled to upstream cell surface receptors to enable fast-timescale sensing of extracellular ligands, and downstream connections can regulate gene expression. We engineered cell-based cytokine controllers that dynamically sense and suppress activated T cells. Our work introduces a generalizable approach that allows the design of signaling circuits that enable user-defined sense-and-respond function for diverse biosensing and therapeutic applications.12aLiu, J.;Yang, X.;Rocks, J.;Jiang, K.;Walters, A. J.;Rai, K.;Bashor, C.40uhttps://drive.google.com/file/d/1D1ZHw5J5aWBxtiv5G6BC1dX4rBosltma/view?usp=drive_linkzPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx251009s9999    xx            000 0 und d