03662nam a22004095i 4500001001800000003000900018005001700027007001500044008004100059020001800100020001900118024003500137082001600172100003000188245010500218264004600323300006500369336002600434337002600460338003600486347002400522505064000546520168801186650001902874650001902893650001402912650001602926650001902942650002702961650001802988650001903006700003603025710003403061773002003095776003603115856010103151978-0-387-78175-4DE-He21320260521092005.0cr nn 008mamaa110409s2011    xxu|    s    |||| 0|eng d  a9780387781754  a997803877817547 a10.1007/978-0-387-78175-42doi04a570.2822231 aPrice, Robert L.eeditor.10aBasic Confocal Microscopyh[electronic resource] /cedited by Robert L. Price, W. Gray (Jay) Jerome. 1aNew York, NY :bSpringer New York,c2011.  aXI, 302p. 139 illus., 103 illus. in color.bonline resource.  atextbtxt2rdacontent  acomputerbc2rdamedia  aonline resourcebcr2rdacarrier  atext filebPDF2rda0 aTable of Contents.- Chapter 1, Introduction and Historical Perspective.-Chapter 2, The Theory of Fluorescence.- Chapter 3, Fluorescence Microscopy.- Chapter 4, Specimen Preparation -- Chapter 5,  Labeling Considerations for Confocal Microscopy -- Chapter 6, Introduction to Digital Imaging for Confocal Microscopy -- Chapter 7, Digital Image Capture for Confocal Microscopy -- Chapter 8, Types of Confocal Instruments: Basic Principals and Advantages and Disadvantages.- Chapter 9, Setting the Operating Parameters.- Chapter 10, 3D Reconstruction of Confocal Image Data.- Chapter 11, Ethics and Resources.- Glossary  .  aMost researchers agree that biological confocal microscopy was jump-started by the confocal design first published by White and Amos in 1985 in the Journal of Cell Biology. As a result, this remains a relatively young field. Yet the use of the technique has grown phenomenally since those early efforts, with new users joining the ranks daily. The publication of Basic Confocal Microscopy reflects the burgeoning need to train new students, technologists, and faculty wishing to use confocal microscopy in their research. A direct outgrowth of the authors' five-day intensive course in the subject begun in 2005, this book covers the basics and includes all the information required to design, implement, and interpret the results of, biological experiments based on confocal microscopy. Concise yet comprehensive, the volume begins by covering the core issues of fluorescence, specimen preparation and labeling, before moving on to address the analog-to-digital conversion of specimen data gathered using confocal microscopy. Subsequent chapters detail the practicalities of operating confocal microscopes, providing all the information necessary to begin practicing confocal microscopy as well as optimizing the material obtained. The final block of chapters examine 3-dimensional analysis and the reconstruction of data sets, outline some of the ethical considerations in confocal imaging, and then supply a number of resources that the authors have found useful in their own work. Once readers have mastered the information this book presents, the resources found in its pages will be an excellent guide to continued learning about the more advanced forms of confocal microscopy. 0aLIFE SCIENCES. 0aBIOTECHNOLOGY. 0aCYTOLOGY. 0aMICROSCOPY.14aLIFE SCIENCES.24aBIOLOGICAL MICROSCOPY.24aCELL BIOLOGY.24aBIOTECHNOLOGY.1 aJerome, W. Gray (Jay).eeditor.2 aSpringerLink (Online service)0 tSpringer eBooks08iPrinted edition:z978038778174740uhttp://dx.doi.org/10.1007/978-0-387-78175-4zVer el texto completo en las instalaciones del CICY