02579nam a2200325Ia 4500 MX-MdCICY 20260521091814.0 CICY B-21772 Full-length agave transcriptome reveals candidate glycosyltransferase genes involved in hemicellulose biosynthesis International Journal of Biological Macromolecules, 274, p.133508, 2024 Artículo Agave species are typical crassulacean acid metabolism (CAM) plants commonly cultivated to produce beverages, fibers, and medicines. To date, few studies have examined hemicellulose biosynthesis in Agave H11648, which is the primary cultivar used for fiber production. We conducted PacBio sequencing to obtain full-length tran scriptome of five agave tissues: leaves, shoots, roots, flowers, and fruits. A total of 41,807 genes were generated, with a mean length of 2394 bp and an annotation rate of 97.12 percent using public databases. We identified 42 glycosyltransferase genes related to hemicellulose biosynthesis, including mixed-linkage glucan (1), gluco mannan (5), xyloglucan (16), and xylan (20). Their expression patterns were examined during leaf development and fungal infection, together with hemicellulose content. The results revealed four candidate glycosyl transferase genes involved in xyloglucan and xylan biosynthesis, including glucan synthase (CSLC), xylosyl transferase (XXT), xylan glucuronyltransferase (GUX), and xylan α-1,3-arabinosyltransferase (XAT). These genes can be potential targets for manipulating xyloglucan and xylan traits in agaves, and can also be used as candidate enzymatic tools for enzyme engineering. We have provided the first full-length transcriptome of agave, which will be a useful resource for gene identification and characterization in agave species. We also elucidated the hemicellulose biosynthesis machinery, which will benefit future studies on hemicellulose traits in agave. AGAVE FULL-LENGTH TRANSCRIPTOME GLYCOSYLTRANSFERASE GENE HEMICELLULOSE BIOSYNTHESIS LEAF DEVELOPMENT FUNGUS INFECTION Huang, X Hu, X. Liu, Q. Xie, Z. Tan, S. Qin, X. Yi, K. https://drive.google.com/file/d/1AgVQ-KfeOrlWmzbuKMVtKIFdjb0RMvNP/view?usp=drive_link Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 31773 31773 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-26 0 B-21772 2025-06-26 09:31:25 2025-06-26 REF1