02279nam a2200325Ia 4500 MX-MdCICY 20260521091809.0 CICY B-21615 Organization and expression of two Arabidopsis DREB2 genes encoding DRE-binding proteins involved in dehydration- and high-salinity-responsive gene expression Plant Molecular Biology, 42, p.657-665, 2000 Artículo In plants, a cis-acting element, DRE/CRT, is involved in ABA-independent gene expression in response to dehydration and low-temperature stress. To understand signal transduction pathways from perception of the dehydration stress signal to gene expression, we characterized a gene family for DRE/CRT-binding proteins DREB2A and DREB2B in Arabidopsis thaliana. Northern analysis showed that both genes are induced by dehydration and high-salt stress. Organ-specific northern analysis with gene-specific probes showed that these genes are strongly induced in roots by high-salt stress and in stems and roots by dehydration stress. The DREB2A gene is located on chromosome 5, and DREB2B on chromosome 3. We screened an Arabidopsis genomic DNA library with cDNA fragments of DREB2A and DREB2B as probes, and isolated DNA fragments that contained 50 -flanking regions of these genes. Sequence analysis showed that both genes are interrupted by a single intron at identical positions in their leader sequence. Several conserved sequences were found in the promoter regions of both genes. The β-glucuronidase (GUS) reporter gene driven by the DREB2 promoters was induced by dehydration and high-salt stress in transgenic Arabidopsis plants. DREB DRE ARABIDOPSIS DEHYDRATION HIGH SALT STRESS PROMOTER Nakashima, K. Shinwari, Z. K. Sakuma, Y. Seki, M. Miura, S. Shinozaki, K. Yamaguchi-Shinozaki, K.  https://drive.google.com/file/d/151oz1IWxRimnAsRdmORutzkczqxfvYk-/view?usp=drive_link Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 31622 31622 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-26 0 B-21615 2025-06-26 08:33:36 2025-06-26 REF1