02520nam a2200337Ia 4500 MX-MdCICY 20260521091809.0 CICY B-21589 A module centered on the transcription factor Msn2 from arbuscular mycorrhizal fungus Rhizophagus irregularis regulates drought stress tolerance in the host plant New Phytologist, 240, p.1497-1518, 2023 Artículo Arbuscular mycorrhizal (AM) fungi can form mutualistic endosymbiosis with > 70% of land plants for obtaining fatty acids and sugars, in return, AM fungi promote plant nutrients and water acquisition to enhance plant fitness. However, how AM fungi orchestrate its own signaling components in response to drought stress remains elusive. Here, we identify a transcription factor containing C2H2 zinc finger domains, RiMsn2 from Rhizophagus irregularis. To characterize the RiMsn2, we combined heterologous expression, subcellular localization in yeasts, and biochemical and molecular studies with reverse genetics approaches during the in planta phase. The results indicate that RiMsn2 is highly conserved across AM fungal species and induced during the early stages of symbiosis. It is significantly upregulated in mycorrhizal roots under severe drought conditions. The nucleus-localized RiMsn2 regulates osmotic homeostasis and trehalose contents of yeasts. Importantly, gene silencing analyses indicate that RiMsn2 is essential for arbuscule formation and enhances plant tolerance to drought stress. Results from yeasts and biochemical experiments suggest that the RiHog1-RiMsn2-STREs module controls the drought stress-responsive genes in AM fungal symbiont. In conclusion, our findings reveal that a module centered on the transcriptional activator RiMsn2 from AM fungus regulates drought stress tolerance in host plant. ARBUSCULAR MYCORRHIZAL FUNGI DROUGHT STRESS RHIZOPHAGUS IRREGULARIS RIHOG1 RIMSN2 STRE-CONTROLLED GENES TRANSCRIPTION FACTOR Fan, X. Xie, H. Huang, X. Zhang, S. Nie, Y. Chen, H. Tang, M. https://drive.google.com/file/d/1hUhx7q0rIzXHtYlrFXh9FhXYpwgg5EHy/view?usp=drive_link Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 31597 31597 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-26 0 B-21589 2025-06-26 08:33:35 2025-06-26 REF1