02330nam a2200349Ia 4500 MX-MdCICY 20260521091806.0 CICY B-19703 WAV E3 ubiquitin ligases mediate degradation of IAA32/34 in the TMK1-mediated auxin signaling pathway during apical hook development Proc. Natl. Acad. Sci., 121, p.e2314353121, 2024 Artículo Auxin regulates plant growth and development through downstream signaling pathways, including the best-known SCFTIR1/AFB-Aux/IAA-ARF pathway and several other less characterized "noncanonical" pathways. Recently, one SCFTIR1/AFB-independent noncanonical pathway, mediated by Transmembrane Kinase 1 (TMK1), was discovered through the analyses of its functions in Arabidopsis apical hook development. Asymmetric accumulation of auxin on the concave side of the apical hook triggers DAR1-catalyzed release of the C-terminal of TMK1, which migrates into the nucleus, where it phosphorylates and stabilizes IAA32/34 to inhibit cell elongation, which is essential for full apical hook formation. However, the molecular factors mediating IAA32/34 degradation have not been identified. Here, we show that proteins in the CYTOKININ INDUCED ROOT WAVING 1 (CKRW1)/WAVY GROWTH 3 (WAV3) subfamily act as E3 ubiquitin ligases to target IAA32/34 for ubiquitination and degradation, which is inhibited by TMK1c-mediated phosphorylation. This antagonistic interaction between TMK1c and CKRW1/WAV3 subfamily E3 ubiquitin ligases regulates IAA32/34 levels to control differential cell elongation along opposite sides of the apical hook. WAVY GROWTH 3 RING-FINGER E3 UBIQUITIN LIGASE AUX/IAA PROTEINS TRANSMEMBRANE KINASE 1 NONCANONICAL AUXIN SIGNALING Wang, J.-L. Wang, M. Zhang, L. Li, Y.-X. Li, J.-J. Li, Y.-Y. Pu, Z.-X. Li, D.-Y. Liu, X.-N. Guo, W. https://drive.google.com/file/d/1VF3upb-Q5WmRlXzpBQ71ybXzY9XX_-9I/view?usp=drive_link Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 31506 31506 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-26 0 B-19703 2025-06-26 08:09:25 2025-06-26 REF1