02252nam a2200313Ia 4500003001000000005001700010040000900027245013700036490005300173500001500226520122200241650001801463650003601481650002101517650002701538650003401565700001601599700001301615700001401628700001401642700001401656700001401670700001401684700001401698700001501712700001201727856015801739008004101897MX-MdCICY20260521091806.0  cCICY10aWAV E3 ubiquitin ligases mediate degradation of IAA32/34 in the TMK1-mediated auxin signaling pathway during apical hook development0 aProc. Natl. Acad. Sci., 121, p.e2314353121, 2024  aArtículo3 aAuxin regulates plant growth and development through downstream signaling pathways, including the best-known SCFTIR1/AFB-Aux/IAA-ARF pathway and several other less characterized "noncanonical" pathways. Recently, one SCFTIR1/AFB-independent noncanonical pathway, mediated by Transmembrane Kinase 1 (TMK1), was discovered through the analyses of its functions in Arabidopsis apical hook development. Asymmetric accumulation of auxin on the concave side of the apical hook triggers DAR1-catalyzed release of the C-terminal of TMK1, which migrates into the nucleus, where it phosphorylates and stabilizes IAA32/34 to inhibit cell elongation, which is essential for full apical hook formation. However, the molecular factors mediating IAA32/34 degradation have not been identified. Here, we show that proteins in the CYTOKININ INDUCED ROOT WAVING 1 (CKRW1)/WAVY GROWTH 3 (WAV3) subfamily act as E3 ubiquitin ligases to target IAA32/34 for ubiquitination and degradation, which is inhibited by TMK1c-mediated phosphorylation. This antagonistic interaction between TMK1c and CKRW1/WAV3 subfamily E3 ubiquitin ligases regulates IAA32/34 levels to control differential cell elongation along opposite sides of the apical hook.14aWAVY GROWTH 314aRING-FINGER E3 UBIQUITIN LIGASE14aAUX/IAA PROTEINS14aTRANSMEMBRANE KINASE 114a NONCANONICAL AUXIN SIGNALING12aWang, J.-L.12aWang, M.12aZhang, L.12aLi, Y.-X.12aLi, J.-J.12aLi, Y.-Y.12aPu, Z.-X.12aLi, D.-Y.12aLiu, X.-N.12aGuo, W.40uhttps://drive.google.com/file/d/1VF3upb-Q5WmRlXzpBQ71ybXzY9XX_-9I/view?usp=drive_linkzPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx250602s9999    xx |||||s2   |||| ||und|d