Zhang Y. Dai M. Wu Z. Wang S. Fan Y. Ni K. Lu X. Liu X. Liu M. Chen W. Chen X. Wang D. Wang J. Guo L. Zhao L. Wang X. Ye W. text xx 9999 monographic und

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As a receptor for plant melatonin, CAND2/PMTR plays an important role in melatonin signaling. Most of the CANDs are membrane proteins and play indispensable roles in signal transduction. In this study, the CANDs from four cotton species were characterized, and the phylogenetic relationships, expression patterns, stress responses of cotton CANDs were analyzed by bioinformatics. Through the analysis of phylogenetic and protein structure, it was found that the CANDs in clade ? might function as cotton melatonin receptors, and most of the GhCANDs in clade ? were induced by melatonin. A putative cotton melatonin receptor, GhCAND2-D5, was functionally probed by gene silencing. The plants with silenced expression of this gene exhibited decreased salt tolerance. Protein interaction prediction identified that GhCAND2-D5 interacted with several membrane proteins and played an important role in melatonin signaling. This study provided a theoretical reference for further investigation of melatonin signaling in cotton. © 2023 Elsevier Masson SAS ABIOTIC STRESS CANDIDATE G-PROTEIN COUPLED RECEPTOR COTTON MELATONIN PLANT MELATONIN RECEPTORS https://drive.google.com/file/d/1cIPeM9v_wDH9GuiEkmPyPDnIDi-ddMln/view?usp=drivesdk https://drive.google.com/file/d/1cIPeM9v_wDH9GuiEkmPyPDnIDi-ddMln/view?usp=drivesdk 250602 20260521091758.0