02581nam a2200325Ia 4500 MX-MdCICY 20260521091756.0 CICY B-21207 Applications of chromatographic methods in metabolomics: A review Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 1239, 124124, 2024, DOI: 10.1016/j.jchromb.2024.124124 Chromatography is a robust and reliable separation method that can use various stationary phases to separate complex mixtures commonly seen in metabolomics. This review examines the types of chromatography and stationary phases that have been used in targeted or untargeted metabolomics with methods such as mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy. General considerations for sample pretreatment and separations in metabolomics are considered, along with the various supports and separation formats for chromatography that have been used in such work. The types of liquid chromatography (LC) that have been most extensively used in metabolomics will be examined, such as reversed-phase liquid chromatography and hydrophilic liquid interaction chromatography. In addition, other forms of LC that have been used in more limited applications for metabolomics (e.g., ion-exchange, size-exclusion, and affinity methods) will be discussed to illustrate how these techniques may be utilized for new and future research in this field. Multidimensional LC methods are also discussed, as well as the use of gas chromatography and supercritical fluid chromatography in metabolomics. In addition, the roles of chromatography in NMR- vs. MS-based metabolomics are considered. Applications are given within the field of metabolomics for each type of chromatography, along with potential advantages or limitations of these separation methods. © 2024 GAS CHROMATOGRAPHY LIQUID CHROMATOGRAPHY MASS SPECTROMETRY METABOLOMICS NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY SUPERCRITICAL FLUID CHROMATOGRAPHY Ovbude S.T. Sharmeen S. Kyei I. Olupathage H. Jones J. Bell R.J. Powers R. Hage D.S. https://drive.google.com/file/d/1op-BHjRAVfyFS9NKdP3D6cHoniU_m6ns/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 31217 31217 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-21207 2025-06-25 16:43:51 2025-06-25 REF1