02051nam a2200217Ia 4500003001000000005001700010040000900027245010700036490005600143520129000199650000801489650002201497650001901519650002001538650002501558700001801583700001901601700001801620856015401638008004101792MX-MdCICY20260521091755.0  cCICY10aReduced expression of bZIP19 and bZIP23 increases zinc and cadmium accumulation in Arabidopsis halleri0 vPlant, Cell & Environment, 47(6), p.2093-2108, 20243 aZinc is an essential micronutrient for all living organisms. When challenged by zinc-limiting conditions, Arabidopsis thaliana plants use a strategy centered on two transcription factors, bZIP19 and bZIP23, to enhance the expression of several zinc transporters to improve their zinc uptake capacity. In the zinc and cadmium hyperaccumulator plant Arabidopsis halleri, highly efficient root-to-shoot zinc translocation results in constitutive local zinc deficiency in roots and in constitutive high expression of zinc deficiency-responsive ZIP genes, supposedly boosting zinc uptake and accumulation. Here, to disrupt this process and to analyze the functions of AhbZIP19, AhbZIP23 and their target genes in hyperaccumulation, the genes encoding both transcriptional factors were knocked down using artificial microRNAs (amiRNA). Although AhbZIP19, AhbZIP23, and their ZIP target genes were downregulated, amiRNA lines surprisingly accumulated more zinc and cadmium compared to control lines in both roots and shoot driving to shoot toxicity symptoms. These observations suggested the existence of a substitute metal uptake machinery in A. halleri to maintain hyperaccumulation. We propose that the iron uptake transporter AhIRT1 participates in this alternative pathway in A. halleri.14aZIP14aHYPERACCUMULATION14aMETAL TOXICITY14aMETAL TRANSPORT14aTRANSCRIPTION FACTOR12aSpielmann, J.12aSchloesser, M.12aHanikenne, M.40uhttps://drive.google.com/open?id=1FCPNnXAHu-RDnvlcHUdubzVPsih0wKgk&usp=drive_copyzPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx250602s9999    xx |||||s2   |||| ||und|d