Yutthanasirikul, R. Kurdrid, P. Saree, S. Senachak, J. Saelee, M. Hongsthong, A. text xx 9999 monographic und

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Comparative studies of proteome analysis, photosynthetic activity and fatty acid biosynthesis of the glnA- and glsF-overexpressing strains and the wild-type (WT)were carried out to elucidate the possible role(s)of the two enzymes, glutamine synthetase (GS)and glutamate synthase (GOGAT)in the GS-GOGAT cycle under nitrogen starvation. The evidence indicated the role of GS and GOGAT in the regulatory mechanism of carbon (C-)and nitrogen (N-)assimilation and the induction of photosynthesis and lipid/fatty acid biosynthesis pathway under N-stress. The fatty acid profile of the GS-overexpressing strain (WT + GlnA)under the stress showed a drastic increase in the levels of C18:1?9 and C18:1?11, whereas only C18:1?9 was significantly induced in the GOGAT-overexpressing strain (WT + GlsF). Moreover, the proteome analysis and oxygen evolution data showed that the extra-GS activity in the mutant (MT)cells led to the increasing of proteins involved in photosynthesis as well as the rate of oxygen evolution in the first 24 h after nitrogen depletion, suggesting its role in promoting conversion of light energy to chemical energy under the stress. ENZYMATIC ACTIVITY ARTHROSPIRA PLATENSIS SYNECHOCOCCUS ELONGATUS PCC 7942 FATTY ACID BIOSYNTHESIS PHOTOSYNTHESIS NITROGEN DEPLETION https://drive.google.com/open?id=1hg7uN__msfbNtBdt9ZOvNUOcTd2LS2TD&usp=drive_copy https://drive.google.com/open?id=1hg7uN__msfbNtBdt9ZOvNUOcTd2LS2TD&usp=drive_copy 250602 20260521091752.0