02192nam a2200301Ia 4500 MX-MdCICY 20260521091752.0 CICY B-21055 Increasing activity of the GS-GOGAT cycle highlights the compensation of N-assimilation in the absence of nitrogen and its metabolic effects in cyanobacteria Algal Research, 79, p.103490, 2024 Comparative studies of proteome analysis, photosynthetic activity and fatty acid biosynthesis of the glnA- and glsF-overexpressing strains and the wild-type (WT)were carried out to elucidate the possible role(s)of the two enzymes, glutamine synthetase (GS)and glutamate synthase (GOGAT)in the GS-GOGAT cycle under nitrogen starvation. The evidence indicated the role of GS and GOGAT in the regulatory mechanism of carbon (C-)and nitrogen (N-)assimilation and the induction of photosynthesis and lipid/fatty acid biosynthesis pathway under N-stress. The fatty acid profile of the GS-overexpressing strain (WT + GlnA)under the stress showed a drastic increase in the levels of C18:1?9 and C18:1?11, whereas only C18:1?9 was significantly induced in the GOGAT-overexpressing strain (WT + GlsF). Moreover, the proteome analysis and oxygen evolution data showed that the extra-GS activity in the mutant (MT)cells led to the increasing of proteins involved in photosynthesis as well as the rate of oxygen evolution in the first 24 h after nitrogen depletion, suggesting its role in promoting conversion of light energy to chemical energy under the stress. ENZYMATIC ACTIVITY ARTHROSPIRA PLATENSIS SYNECHOCOCCUS ELONGATUS PCC 7942 FATTY ACID BIOSYNTHESIS PHOTOSYNTHESIS NITROGEN DEPLETION Yutthanasirikul, R. Kurdrid, P. Saree, S. Senachak, J. Saelee, M. Hongsthong, A. https://drive.google.com/open?id=1hg7uN__msfbNtBdt9ZOvNUOcTd2LS2TD&usp=drive_copy Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 31072 31072 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-21055 2025-06-25 16:25:00 2025-06-25 REF1