01818nam a2200241Ia 4500 MX-MdCICY 20260521091741.0 CICY B-20712 Heterologous expression and characterization of Penicillium citrinum nuclease P1 in Aspergillus niger and its application in the production of nucleotides Protein Expression and Purification, 156, p.36-43, 2019 Nuclease P1 gene (nuc P1)which was cloned from Penicillium citrinum and expressed in A percent niger Bdel4 with the low-background extracellular protein percent The expression strategy of multi-copy nuc P1 in the A percent niger with the linker of 2A peptide was applied to improve the enzyme activity of nuclease P1, the highest activity up to 77 percent6 U/mL percent After Ni-chelate purification, the specific enzyme activity, the optimum temperature and pH were 32 percent4 U/mg, 65 °C and 5 percent3 respectively percent The recombination nuclease P1 was activated by addition of Mg2+, Zn2+ and Cu2+, and inhibited by addition of Ca2+, Fe2+, Mn2+, Ni2+, Co2+, Mg2+, K+ and EDTA percent Furthermore, the enzyme hydrolyses yeast RNA efficiently into 5'- nucleotides percent Through enzymolysis, the highest concentration of nucleotides achieved 15 percent12 mg/mL, and 75U nuclease P1 is suitable amount should be added to the enzymolysis system percent NUCLEASE P1 2A PEPTIDE ASPERGILLUS NIGER RECOMBINANT EXPRESSION Chen, X. Wang, B. Pan, L. https://drive.google.com/file/d/1cdWXN6i5MW7SVTdg7BpPxfXHslcZx-zd/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 30733 30733 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-20712 2025-06-25 16:24:54 2025-06-25 REF1