02255nam a2200265Ia 4500 MX-MdCICY 20260521091740.0 CICY B-20700 Cloning and characterization of a novel carotenoid cleavage dioxygenase 1 from Helianthus annuus Chemistry & BioDiversity, 19(1), p.e202100694, 2022 Natural ?-ionone, a high-value flavoring agent, has been widely applied in the food, cosmetics, and perfume industry. However, attempts to overproduce ?-ionone in microorganisms have been limited by the efficiency of carotenoid cleavage dioxygenases (CCDs), which catalyzes ?-carotene in the biosynthesis pathway. In order to obtain CCD genes responsible for the specific cleavage of carotenoids generating ?-ionone, a novel carotenoid cleavage dioxygenase 1 from Helianthus annuus was cloned and overexpressed in Escherichia coli BL21(DE3). The recombinant CCD was able to cleave a variety of carotenoids at the 9, 10 (9', 10')sites to produce C13 products in vitro, including ?-ionone, pseudoionone, 3-hydroxy-4-oxo-?-ionone, 3-hydroxy-?-ionone, and 3-hydroxy-?-ionone, which vary depending on the carotenoid substrates. In comparison with lycopene and zeaxanthin, HaCCD1 also showed the high specificity for ?-carotene to cleave the 9, 10 (9', 10')double bond to produce ?-ionone in E. coli accumulating carotenoids. Finally, the expression of HaCCD1 in E. coli was optimized, and biochemical characterizations were further clarified. The optimal activity of HaCCD1 was at pH 8.8 and 50 °C. The Vmax for ?-apo-8'-carotenal was 10.14 U/mg, while the Km was 0.32 mM. Collectively, our study provides a valuable enzyme for the synthesis of natural ?-ionone by biotransformation and synthetic biology platform. CCD1 HELIANTHUS ANNUUS CAROTENOID CLEAVAGE DIOXYGENASES B-IONONE Qi, Z. Tong, X. Bu, S. Pei, J. Zhao, L. https://drive.google.com/file/d/1hVyIrfWUS8oAvvFGA9iEz0mTeUaLxm23/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 30722 30722 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-20700 2025-06-25 16:24:53 2025-06-25 REF1