Wang, J. Yang, C. Wu, X. Wang, Y. Wang, B. Wu, X. Li, G. text xx 9999 monographic und

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As the largest group of plant resistance genes, the nucleotide-binding site-leucine-rich repeat (NBS-LRR)genes play a critical role in plant disease defense system. The bottle gourd is a distinctive horticultural crop of the Cucurbitaceae family and powdery mildew is one of the major diseases jeopardizing crop production and quality. In this study, we have identified 84 NBS-LRR genes and divided into seven subfamilies. Phylogenetic tree and motif analysis grouped these genes into two major clusters with toll/interleukin-1 like receptor (TIR)domain or a coiled-coil domain. Subsequently, duplication events, motif sequence, orthologous gene cluster, protein-protein interaction, cis-elements were analyzed. 12 pairs of tandem duplicated genes and two pairs of segmental duplicated genes were detected in NBS-LRR family. 14 singleton genes and 62 functional cis-elements were found. The structure of 27 proteins were constructed by homology modeling and divided into five different types. 34 differentially expressed genes of NBS-LRR genes were found in PM-resistant and PM-susceptible line. Expression of NBS-LRR genes were validated by qRT-PCR under PM stress and gene Lsi04g015960 with powdery mildew8 (RPW8)domain was significantly up-regulated in PM-resistant variety. Our study provides fundamental insights of NBS-LRR family genes in bottle gourd and find a potential candidate gene Lsi04g015960 for PM tolerance breeding. BOTTLE GOURD NBS-LRR FAMILY PHYLOGENETIC ANALYSIS POWDERY MILDEW EXPRESSION ANALYSIS https://drive.google.com/file/d/1Qza84hGz1tMg2ATYTDI_YLswWCe-cfRc/view?usp=drivesdk https://drive.google.com/file/d/1Qza84hGz1tMg2ATYTDI_YLswWCe-cfRc/view?usp=drivesdk 250602 20260521091738.0