02407nam a2200301Ia 4500 MX-MdCICY 20260521091738.0 CICY B-20626 Genome-wide characterization of NBS-LRR family genes and expression analysis under powdery mildew stress in Lagenaria siceraria Physiological and Molecular Plant Pathology, 118, p.101798, 2022 As the largest group of plant resistance genes, the nucleotide-binding site-leucine-rich repeat (NBS-LRR)genes play a critical role in plant disease defense system. The bottle gourd is a distinctive horticultural crop of the Cucurbitaceae family and powdery mildew is one of the major diseases jeopardizing crop production and quality. In this study, we have identified 84 NBS-LRR genes and divided into seven subfamilies. Phylogenetic tree and motif analysis grouped these genes into two major clusters with toll/interleukin-1 like receptor (TIR)domain or a coiled-coil domain. Subsequently, duplication events, motif sequence, orthologous gene cluster, protein-protein interaction, cis-elements were analyzed. 12 pairs of tandem duplicated genes and two pairs of segmental duplicated genes were detected in NBS-LRR family. 14 singleton genes and 62 functional cis-elements were found. The structure of 27 proteins were constructed by homology modeling and divided into five different types. 34 differentially expressed genes of NBS-LRR genes were found in PM-resistant and PM-susceptible line. Expression of NBS-LRR genes were validated by qRT-PCR under PM stress and gene Lsi04g015960 with powdery mildew8 (RPW8)domain was significantly up-regulated in PM-resistant variety. Our study provides fundamental insights of NBS-LRR family genes in bottle gourd and find a potential candidate gene Lsi04g015960 for PM tolerance breeding. BOTTLE GOURD NBS-LRR FAMILY PHYLOGENETIC ANALYSIS POWDERY MILDEW EXPRESSION ANALYSIS Wang, J. Yang, C. Wu, X. Wang, Y. Wang, B. Wu, X. Li, G. https://drive.google.com/file/d/1Qza84hGz1tMg2ATYTDI_YLswWCe-cfRc/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 30651 30651 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-20626 2025-06-25 16:24:52 2025-06-25 REF1