02326nam a2200313Ia 4500 MX-MdCICY 20260521091736.0 CICY B-20574 Lasiodiplodia regiae sp. nov.: a new species causing canker and dieback of fruit trees in China PhytoPathology, 113(7), p.1210-1221, 2023 Canker and dieback are serious fungal diseases of woody plants that can cause huge economic losses to orchards. The purpose of this study was to classify and assess the pathogenicity of fungal species associated with canker and dieback on fruit trees growing in Henan Province, China. In total, 150 isolates of Botryosphaeriaceae were obtained from six different fruit trees exhibiting typical symptoms of stem canker, branch dieback, and gummosis. Morphological examinations and phylogenetic analysis of ITS, tef1, tub2, and rpb2 revealed two Botryosphaeriaceae species, which are Botryosphaeria dothidea and a novel species, Lasiodiplodia regiae, respectively. Using Koch's postulates, we confirmed that the different isolates of L. regiae can cause disease in their original hosts. The pathogenicity tests showed that L. regiae can cause canker, dieback, and gummosis symptoms in four different hosts, indicating a relatively wider host range. Moreover, 10 L. regiae isolates exhibited similar symptoms but different levels of virulence on shoots of peach trees under field conditions. This study demonstrated that L. regiae was a new causal agent of canker and dieback of six fruit tree species, which could be a serious risk to the orchard industry in China. Furthermore, the findings provide a foundation for further epidemiological studies and the development of management strategies. BOTRYOSPHAERIACEAE LASIODIPLODIA REGIAE CANKER DIEBACK FRUIT TREES PATHOGENICITY Wang, Y. Xie, S. Cao, J. Zhao, H. Yin, X. Guo, Y. Zhang, M. https://drive.google.com/file/d/1W6nWSl_U4asxcqnwxKtCAhkIBe1TU7S4/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 30599 30599 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-20574 2025-06-25 16:24:51 2025-06-25 REF1