02468nam a2200325Ia 4500003001000000005001700010040000900027090001200036245009500048490006400143520132400207650001801531650002101549650001201570650002101582650002601603650002701629700002901656700001501685700002301700700002501723700002201748700001801770700002301788856015601811942001401967008004101981999001702022952010302039MX-MdCICY20260521091729.0  cCICY  aB-2030910aTranscriptional memory contributes to drought tolerance in coffee (Coffea canephora)plants0 vEnvironmental and Experimental Botany, 147, p.220-233, 20183 aWater deprivation is an important limiting factor in the productivity of crops like coffee. In addition to transcription factors (TFs)and small non-coding RNAs, transcriptional memory seems to act in gene expression modulation during plant drought response. Here, a RNA-Seq approach was used to investigate the drought responses of Coffea canephora clones 109 and 120, which are respectively sensitive and tolerant to drought. Illumina sequencing allowed us to identify differentially expressed genes (DEG)in the tolerant (826)and sensitive (135)clones and their enriched categories. Our results indicate that the sensitive clone may trigger an oxidative stress response, possibly leading to programmed cell death, when exposed to multiple drought episodes. The acclimation of tolerant plants, on the other hand, seems to involve antioxidant secondary metabolism and the ABA response. Most importantly, 49 memory genes were identified in the tolerant clone. They were mainly linked to the ABA pathway, protein folding and biotic stress. Small RNA profiling also identified regulatory microRNAs in coffee leaves, including hundreds of putative novel ones. Our findings strongly suggest that transcriptional memory modulates the expression of drought-responsive genes and contributes to drought tolerance in C. canephora.14aABSCISIC ACID14aCOFFEA CANEPHORA14aDROUGHT14aOXIDATIVE STRESS14aRECEPTOR-LIKE KINASES14aTRANSCRIPTIONAL MEMORY12aDe Freitas Guedes, F. A.12aNobres, P.12aFerreira, D. C. R.12aMenezes-Silva, P. E.12aRibeiro-Alves, M.12aCorrea, R. L.12aAlves-Ferreira, M.40uhttps://drive.google.com/file/d/1HQvy1rt4PqO66PAlQ5HGB40-nxGxeGuB/view?usp=drivesdkzPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx  2LoccREF1250602s9999    xx |||||s2   |||| ||und|d  c30345d30345  00102Loc40708F1aCICYbCICYcREd2025-06-25l0oB-20309r2025-06-25 16:24:46w2025-06-25yREF1