01805nam a2200265Ia 4500 MX-MdCICY 20260521091719.0 CICY B-20000 Live-cell imaging reveals the cellular dynamics in seed development Plant Science, 325, p.111485, 2022 Seed development in flowering plants is highly complex and governed by three genetically distinct tissues: the fertilization products, the diploid embryo and triploid endosperm, as well as the seed coat that has maternal origin. There are diverse cellular dynamics such as nuclear movement in gamete cells for fertilization, cell polarity establishment for embryo development, and multinuclear endosperm formation. These tissues also coordinate and synchronize the developmental timing for proper seed formation through cell-to-cell communications. Live-cell imaging using advanced microscopy techniques enables us to decipher the dynamics of these events. Especially, the establishment of a less-invasive semi-in vivo live-cell imaging approach has allowed us to perform time-lapse analyses for long period observation of Arabidopsis thaliana intact seed development dynamics. Here we highlight the recent trends of live-cell imaging for seed development and discuss where we are heading. SEMI-IN VIVO LIVE-CELL IMAGING SEED CONFOCAL MICROSCOPY FERTILIZATION EMBRYO ENDOSPERM Shin, J. M. Yuan, L. Kawashima, T. https://drive.google.com/file/d/1Kg7uSUB_6YUubWVOSrfj4SQOOsbRxRDb/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 30044 30044 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-20000 2025-06-25 16:24:40 2025-06-25 REF1