01726nam a2200229Ia 4500003001000000005001700010040000900027245007200036490003900108520099400147650003501141650000901176650002401185650001801209650001101227650001401238700001601252700001301268700001801281856015601299008004101455MX-MdCICY20260521091719.0  cCICY10aLive-cell imaging reveals the cellular dynamics in seed development0 vPlant Science, 325, p.111485, 20223 aSeed development in flowering plants is highly complex and governed by three genetically distinct tissues: the fertilization products, the diploid embryo and triploid endosperm, as well as the seed coat that has maternal origin. There are diverse cellular dynamics such as nuclear movement in gamete cells for fertilization, cell polarity establishment for embryo development, and multinuclear endosperm formation. These tissues also coordinate and synchronize the developmental timing for proper seed formation through cell-to-cell communications. Live-cell imaging using advanced microscopy techniques enables us to decipher the dynamics of these events. Especially, the establishment of a less-invasive semi-in vivo live-cell imaging approach has allowed us to perform time-lapse analyses for long period observation of Arabidopsis thaliana intact seed development dynamics. Here we highlight the recent trends of live-cell imaging for seed development and discuss where we are heading.14aSEMI-IN VIVO LIVE-CELL IMAGING14aSEED14aCONFOCAL MICROSCOPY14aFERTILIZATION14aEMBRYO14aENDOSPERM12aShin, J. M.12aYuan, L.12aKawashima, T.40uhttps://drive.google.com/file/d/1Kg7uSUB_6YUubWVOSrfj4SQOOsbRxRDb/view?usp=drivesdkzPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx250602s9999    xx |||||s2   |||| ||und|d