02154nam a2200313Ia 4500 MX-MdCICY 20260521091718.0 CICY B-19981 Comparative transcriptomic and metabolomic analysis revealed molecular mechanism of two wheat near-isogenic lines response to nitrogen application 30(195), p.47-572022 Nitrogen (N)is an essential nutrient element required for plant growth, and the development of wheat varieties with high nitrogen use efficiency (NUE)is an urgent need for sustainable crop production. However, the molecular mechanism of NUE between diverse wheat varieties in response to N application remains unclear. To reveal the possible molecular mechanisms underlying this complex phenomenon, we investigated the transcriptional and metabolic changes of flag leaves of two wheat near-isogenic lines (NILs)differing in NUE under two N fertilizer treatments. Comparative transcriptome analysis indicated that the expression levels of the genes responsible for carbon and nitrogen metabolism were significantly higher in high-NUE wheat. The metabolome comparison revealed that the activity of the tricarboxylic acid (TCA)cycle was enhanced in high-NUE wheat, while reduced in low-NUE wheat after the N fertilizer application. Additionally, amino acid metabolism increased in both wheat NILs but more increased in high-NUE wheat. In summary, more upregulated transcripts and metabolites were identified in high-NUE wheat, and this study provides valuable new insights for improving NUE in wheat. CARBON METABOLISM METABOLOME NITROGEN NITROGEN METABOLISM TRANSCRIPTOME WHEAT Zhang, X. Ding, Y. Ma, Q. Li, F. Tao, R. Li, T. Zhu, X. https://drive.google.com/file/d/1O6HV6dFw1h310R1fz9bQREF9WO2f_Wz6/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 30025 30025 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-19981 2025-06-25 16:24:40 2025-06-25 REF1