02304nam a2200277Ia 4500 MX-MdCICY 20260521091714.0 CICY B-19838 Extraction of hyaluronic acid (HA)from rooster comb and characterization using flow field-flow fractionation (FlFFF)coupled with multiangle light scattering (MALS) Journal of separation Science, 33(22), p.3530-3536, 2010 Hyaluronic acid (HA)was extracted in a relatively large scale from rooster comb using a method similar to that reported previously. The extraction method was modified to simplify and to reduce time and cost in order to accommodate a large-scale extraction. Five hundred grams of frozen rooster combs yielded about 500 mg of dried HA. Extracted HA was characterized using asymmetrical flow field-flow fractionation (AsFlFFF)coupled online to a multiangle light scattering detector and a refractive index detector to determine the molecular size, molecular weight (MW)distribution, and molecular conformation of HA. For characterization of HA, AsFlFFF was operated by a simplified two-step procedure, instead of the conventional three-step procedure, where the first two steps (sample loading and focusing)were combined into one to avoid the adsorption of viscous HA onto the channel membrane. The simplified two-step AsFlFFF yielded reasonably good separations of HA molecules based on their MWs. The weight average MW (M(w)) and the average root-mean-square (RMS)radius of HA extracted from rooster comb were 1.20×10(6)and 94.7 nm, respectively. When the sample solution was filtered through a 0.45 ?m disposable syringe filter, they were reduced down to 3.8×10(5)and 50.1 nm, respectively. ASYMMETRICAL FLOW FIELD-FLOW FRACTIONATION HYALURONIC ACID MULTIANGLE LIGHT SCATTERING MOLECULAR WEIGHT DISTRIBUTION ROOSTER COMB Kang, D. Y. Kim, W. S. Heo, I. S. Park, Y. H. Lee, S. https://drive.google.com/file/d/1uZzyoyGRpZYFOEey6zqK5obZ6Z3q9B0p/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 29893 29893 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-19838 2025-06-25 16:24:38 2025-06-25 REF1