02276nam a2200313Ia 4500 MX-MdCICY 20260521091713.0 CICY B-19823 The S40 family members delay leaf senescence by promoting cytokinin synthesis Plant Physiology and BioChemistry, 191, p.99-109, 2022 Leaf senescence is regulated by both endogenous hormones and environmental stimuli in a programmed and concerted way. The members of the S40 family have been reported to play roles in leaf senescence. Here we identified an S40 family member, CiS40-11, from Caragana intermedia. Phylogenetic analysis revealed that the CiS40-11 protein had the highest identity with AtS40-5 (AT1G11700)and AtS40-6 (AT1G61930)of Arabidopsis thaliana. CiS40-11 was highly expressed in leaves and was down-regulated after dark treatment. The subcellular localization analysis showed that CiS40-11 was a cytoplasm-nucleus dual-localized protein. Leaf senescence was delayed in both the CiS40-11 overexpressed A. thaliana and its transiently expressed C. intermedia. Transcriptomic analysis and endogenous hormones assay revealed that CiS40-11 inhibited leaf senescence via promoting the biosynthesis of cytokinins by blocking AtMYB2 expression in the CiS40-11 overexpression lines. Furthermore, overexpression of either AtS40-5 or AtS40-6 showed similar phenotype as the CiS40-11 overexpressing lines, while in the ats40-5a or ats40-6a mutants, the AtMYB2 expression was increased and their leaves exhibited a premature senescence phenotype. These results provide a new molecular mechanism of the S40 family in leaf senescence regulation of plants. ARABIDOPSIS THALIANA ATMYB2 CARAGANA INTERMEDIA CYTOKININS LEAF SENESCENCE SENESCENCE REGULATOR (S40) Yang, T. Zhang, M. Yang, Q. Liu, K. Cui, J. Chen, J. Li, G. https://drive.google.com/file/d/1Bwkkm4mjsmODo0-6np7CK80NohVfpm-K/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 29880 29880 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-19823 2025-06-25 16:24:37 2025-06-25 REF1