Wang, T. Guo, Q. Yang, H. Gao, W. Li, P. text xx 9999 monographic und

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In this study, we developed an efficient and green pH-controlled reversible deep-eutectic solvents (DESs)based enzyme system for efficient extraction, transformation and in-situ separation of natural products from herbs. As a case study, seven isoflavones in the root of Pueraria lobata were selected as target compounds. A series of DESs were firstly prepared and the extraction efficiency for target compounds with or without cellulase catalysis was investigated. We found that [N4444][Cl]:ethylene glycol (1:2)with cellulase addition show the highest extraction efficiency for isoflavones. More importantly, the phase behavior of [N4444][Cl]:ethylene glycol (1:2)based cellulase system was found to be pH-controlled. When pH is 5.0, the system is a homogeneous single-phase system, which is conducive to the extraction of natural products. When pH is 6.4, the system changes from the single-phase to the heterogeneous two-phase, to realize the in-situ separation of the compounds. Therefore, by adjusting the pH of the system, the in-situ separation of isoflavones was successfully realized with high recovery yields. The pH-controlled DESs based enzyme-assisted extraction system overcomes the incompatibility between enzyme catalysis and conventional organic solvent in extraction, which could efficiently extract seven isoflavones from Pueraria lobata root. DEEP-EUTECTIC SOLVENTS CELLULASE ULTRASOUND PH-CONTROLLED SOLVENT SYSTEM PUERARIA LOBATA https://drive.google.com/file/d/1YCrSD-jJcPvTpayUJtbYyHmmeUOu0jUX/view?usp=drivesdk https://drive.google.com/file/d/1YCrSD-jJcPvTpayUJtbYyHmmeUOu0jUX/view?usp=drivesdk 250602 20260521091655.0