Mi, J. Liew, K. X. Al-Babili, S. text xx 9999 monographic und

electronic

Carotenoid oxidative cleavage products, apocarotenoids (APOs), are a class of important plant secondary metabolites, which include phytohormones abscisic acid (ABA)and strigolactones (SLs), and growth regulators and signaling molecules such as ?-cyclocitral, zaxinone, anchorene, ?-apo-11-carotenoids, and retinal. Qualitative and quantitative analysis of these bioactive compounds is crucial for understanding their metabolism and may also enable discovering further regulatory APOs. The state-of-the-art mass spectrometry (MS)technology has advanced the detection of plant APOs; however, it is still challenging to perform an accurate analysis of the low-level phytohormones ABA and SL and the structurally diverse APOs from complex plant matrices. Here, we describe ultrahigh-performance liquid chromatography-MS (UHPLC-MS)methods to determine carotenoid-derived hormones and APOs from plants by integrating ultrasound-assisted extraction and solid-phase extraction. These assays enable an accurate quantification of carotenoid-derived hormones and APOs from plant tissues by using an UHPLC hybrid quadrupole-Orbitrap mass spectrometer. ABSCISIC ACID APOCAROTENOIDS RICE ROOT SOLID-PHASE EXTRACTION STRIGOLACTONES ULTRASOUND-ASSISTED EXTRACTION ULTRAHIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY https://drive.google.com/file/d/1iXfIAnefx2dJwbCOYnGtcRpKEsDw2dbh/view?usp=drivesdk https://drive.google.com/file/d/1iXfIAnefx2dJwbCOYnGtcRpKEsDw2dbh/view?usp=drivesdk 250602 20260521091649.0