02118nam a2200277Ia 4500 MX-MdCICY 20260521091649.0 CICY B-19055 Ultrahigh-Performance Liquid Chromatography-Mass Spectrometry Analysis of Carotenoid-Derived Hormones and Apocarotenoids in Plants Current Protocols, 2(2), p.e375, 2022 Carotenoid oxidative cleavage products, apocarotenoids (APOs), are a class of important plant secondary metabolites, which include phytohormones abscisic acid (ABA)and strigolactones (SLs), and growth regulators and signaling molecules such as ?-cyclocitral, zaxinone, anchorene, ?-apo-11-carotenoids, and retinal. Qualitative and quantitative analysis of these bioactive compounds is crucial for understanding their metabolism and may also enable discovering further regulatory APOs. The state-of-the-art mass spectrometry (MS)technology has advanced the detection of plant APOs; however, it is still challenging to perform an accurate analysis of the low-level phytohormones ABA and SL and the structurally diverse APOs from complex plant matrices. Here, we describe ultrahigh-performance liquid chromatography-MS (UHPLC-MS)methods to determine carotenoid-derived hormones and APOs from plants by integrating ultrasound-assisted extraction and solid-phase extraction. These assays enable an accurate quantification of carotenoid-derived hormones and APOs from plant tissues by using an UHPLC hybrid quadrupole-Orbitrap mass spectrometer. ABSCISIC ACID APOCAROTENOIDS RICE ROOT SOLID-PHASE EXTRACTION STRIGOLACTONES ULTRASOUND-ASSISTED EXTRACTION ULTRAHIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY Mi, J. Liew, K. X. Al-Babili, S. https://drive.google.com/file/d/1iXfIAnefx2dJwbCOYnGtcRpKEsDw2dbh/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 29134 29134 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-19055 2025-06-25 16:24:24 2025-06-25 REF1