01809nam a2200241Ia 4500003001000000005001700010040000900027245004700036490004800083520105300131650003101184650001701215650003001232700001501262700001601277700001501293700001701308700001601325700001601341700001301357856015601370008004101526MX-MdCICY20260521091644.0  cCICY10aChloroplast transformation in oilseed rape0 vTransgenic Research, 12(1), p.111-114, 20033 aThe chloroplast transformation vector pNRAB carries two expression cassettes for the spectinomycin resistance gene aadA and the insect resistance gene cry1Aa10. The two cassettes are sited between the rps7 and ndhB targeting fragments. Biolistic delivery of the vector DNA, followed by spectinomycin selection, yielded chloroplast transformants at a frequency of four in 1000 bombarded cotyledon petioles. PCR analysis and Southern blot of PCR products confirmed the site-specific integration of aadA and cry1Aa10 into the chloroplast genomes of transgenic oilseed rape. When transgenic oilseed rape leaves were fed to second instar Plutella xylostera larvae, 47 percent mortality was observed against this insect and the surviving larvae had significantly lower weight than the control. This is the first report of chloroplast transformation in oilseed rape and the introduction of novel genes between the rps7 and ndhB genes in the chloroplast genome. This offers an opportunity for improvement of oilseed rape by chloroplast genetic engineering.14aCHLOROPLAST TRANSFORMATION14aOILSEED RAPE14aSITE-SPECIFIC INTEGRATION12aHou, B. K.12aZhou, Y. H.12aWan, L. H.12aZhang, Z. L.12aShen, G. F.12aChen, Z. H.12aHu, Z. M40uhttps://drive.google.com/file/d/1Y5lb4Su_-reKnp8GbosKLccf-hEFQvq-/view?usp=drivesdkzPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx250602s9999    xx |||||s2   |||| ||und|d