01247nam a2200241Ia 4500 MX-MdCICY 20260521091635.0 CICY B-18612 Enzymatic assembly of DNA molecules up to several hundred kilobases. Nature methods, 6(5), p.343-345, 2009 We describe an isothermal, single-reaction method for assembling multiple overlapping DNA molecules by the concerted action of a 5? exonuclease, a DNA polymerase and a DNA ligase. First we recessed DNA fragments, yielding single-stranded DNA overhangs that specifically annealed, and then covalently joined them. This assembly method can be used to seamlessly construct synthetic and natural genes, genetic pathways and entire genomes, and could be a useful molecular engineering tool. DNA MOLECULES Gibson, D. G. Young, L. Chuang, R. Y. Venter, J. C. Hutchison, C. A. Smith, H. O. https://drive.google.com/file/d/12K9Z54da4GzV2cDBdQcdGZN4h0CAxgM2/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 28698 28698 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-18612 2025-06-25 16:24:15 2025-06-25 REF1