02753nam a2200277Ia 4500003001000000005001700010040000900027090001200036245015400048490005500202520164700257700002701904700003001931700003001961700001601991700002802007700002602035700002902061700002902090700002502119856015602144942001402300008004102314999001702355952010302372MX-MdCICY20260521091556.0  cCICY  aB-1740010aIn vitro larvicidal and in vivo anthelmintic effects of Oxalis tetraphylla (Oxalidaceae)hydroalcoholic extract against Haemonchus contortus in lambs.0 vJournal of helminthology, 92(3), p. 309-316., 20183 aThe in vitro larvicidal and in vivo anthelmintic effects of Oxalis tetraphylla hydroalcoholic extract (HE), against Haemonchus contortus in experimentally infected lambs, were assessed. We used a microtitration plate method, comprising the following two stages. Stage 1: 20 ?l of water containing 200 sheathed H. contortus infective larvae (ShHcl)were deposited in every well of three series; then, the series 2 and 3 wells were treated with 80 ?l 1 percent ivermectin and O. tetraphylla HE at 20 mg/ml, respectively. Stage 2: the same procedure was performed replacing the ShHcl with exsheathed larvae (ExShHcl). Evaluations were performed after 24 and 48 h. The total numbers of dead and live larvae were counted. A second experiment evaluated the reduction in nematode egg populations in the faeces of lambs treated orally with the O. tetraphylla HE. The 27 lambs used were divided into Groups 1, 2 and 3 (n = 9), which were administered water (positive control), levamisole 1 M (7.5 mg/kg body weight (BW), as a unique dose)and O. tetraphylla HE (20 mg/kg BW), respectively. The plant HE was administered daily for 8 days. The in vitro assay showed 80.9percent and 86.5percent larval mortality of ShHcl after 24 and 48 h, respectively, while the corresponding mortality values for ExShHcl were 97 and 99percent, respectively. The in vivo assay showed variability in the eggs/gram of faeces (epg)values; however, at the end of the trial, the average reduction in the epg values of the O. tetraphylla HE group was 45.6percent (P < 0.05). Oxalis tetraphylla HE contains compounds that belong to the flavonol group with anthelmintic activity.12aGonzález-Cruz, B. J.12aRodríguez-Labastida, M.12aGonzález-Cortázar, M.12aZamilpa, A.12aLópez-Arellano, M. E.12aAguilar-Marcelino, L.12aGonzález-Garduño, R.12aGonzález-Garduño, R.12aMendoza-De Gives, P.40uhttps://drive.google.com/file/d/1YYhlpWs3HJDI10LKDiXfFFQqUXWpjmAx/view?usp=drivesdkzPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx  2LoccREF1250602s9999    xx |||||s2   |||| ||und|d  c27502d27502  00102Loc40708F1aCICYbCICYcREd2025-06-25l0oB-17400r2025-06-25 16:02:10w2025-06-25yREF1