02730nam a2200373Ia 4500 MX-MdCICY 20260521091543.0 CICY B-16975 ITRAQ protein profile differential analysis between somatic globular and cotyledonary embryos reveals stress, hormone, and respiration involved in increasing plantlet regeneration of Gossypium hirsutum L. Journal of Proteome Research, 14(1), p.268-278, 2015 Somatic embryo development (SED)in upland cotton shows low frequencies of embryo maturation and plantlet regeneration. Progress in increasing the regeneration rate has been limited. Here a global analysis of proteome dynamics between globular and cotyledonary embryos was performed using isobaric tags for relative and absolute quantitation to explore mechanisms underlying SED. Of 6318 proteins identified by a mass spectrometric analysis, 102 proteins were significantly up-regulated and 107 were significantly down-regulated in cotyledonary embryos. The differentially expressed proteins were classified into seven functional categories: stress responses, hormone synthesis and signal transduction, carbohydrate and energy metabolism, protein metabolism, cell wall metabolism, cell transport, and lipid metabolism. KEGG (Kyoto Encyclopedia of Genes and Genomes)analysis showed that stress response, hormone homeostasis, and respiration and photosynthesis were involved in SED. Quantitative real-time PCR analysis confirmed the authenticity and accuracy of the proteomic analysis. Treatment of exogenous hormones showed that abscisic acid and jasmonic acid facilitate SED, whereas gibberellic acid inhibits SED and increases abnormal embryo frequency. Thus, global analysis of proteome dynamics reveals that stress response, hormone homeostasis, and respiration and photosynthesis determined cotton SED. The findings of this research improve the understanding of molecular processes, especially environmental stress response, involved in cotton SED. GOSSYPIUM HIRSUTUM HORMONE ITRAQ PROTEOMICS RESPIRATION SOMATIC EMBRYO DEVELOPMENT STRESS UPLAND COTTON Ge, X. Zhang, C. Wang, Q. Yang, Z. Wang, Y. Zhang, X. Wu, Z. Wu, Z. Wu, J. Li, F. https://drive.google.com/file/d/1ipwCU92xQ2BX88TOnad2HIEh7u4L3KV1/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 27079 27079 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-16975 2025-06-25 16:02:01 2025-06-25 REF1