Arencibia A Molina Pr De La Riva G Selman-Housein G. text xx 9999 monographic und

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We describe an efficient procedure for genetic transformation of commercial sugarcane varieties POJ 2878 and Ja 60-5. The transformation protocol is based on electroporation of a plasmid conferring GUS activity into cell clusters isolated from embryogenic calli. Six to eight weeks after electroporation, Ja 60-5 plants regenerated from electroporated tissues were tested and confirmed to be transgenic using histochemical glucuronidase and Southern hybridization analysis. Electroporation of intact cells is an efficient and reproducible method for sugarcane transformation and may also be useful for transformation of other plants. SUGARCANE ELECTROPORATION GUS STABLE TRANSFORMATION https://drive.google.com/file/d/10js3hkezESnJBZiQ8eeEtuiLXhP5xWMj/view?usp=drivesdk https://drive.google.com/file/d/10js3hkezESnJBZiQ8eeEtuiLXhP5xWMj/view?usp=drivesdk 250602 20260521091541.0