01452nam a2200253Ia 4500 MX-MdCICY 20260521091541.0 CICY B-16919 Production of transgenic sugarcane (Saccharum officinarum L.)plants by intact cell electroporation Plant Cell Reports, 14(5):305-309, 1995 We describe an efficient procedure for genetic transformation of commercial sugarcane varieties POJ 2878 and Ja 60-5. The transformation protocol is based on electroporation of a plasmid conferring GUS activity into cell clusters isolated from embryogenic calli. Six to eight weeks after electroporation, Ja 60-5 plants regenerated from electroporated tissues were tested and confirmed to be transgenic using histochemical glucuronidase and Southern hybridization analysis. Electroporation of intact cells is an efficient and reproducible method for sugarcane transformation and may also be useful for transformation of other plants. SUGARCANE ELECTROPORATION GUS STABLE TRANSFORMATION Arencibia A Molina Pr De La Riva G Selman-Housein G. https://drive.google.com/file/d/10js3hkezESnJBZiQ8eeEtuiLXhP5xWMj/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 27023 27023 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-16919 2025-06-25 16:02:00 2025-06-25 REF1