TY  - BOOK
AU  - Sparkes,I.A.
AU  - Runions,J.
AU  - Kearns,A.
AU  - Hawes,C.
TI  - Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants
KW  - ARTICLECELL FUSIÓN
KW  - CELL TRANSFORMATION
KW  - CULTURE TECHNIQUE
KW  - ENDOPLASMIC RETICULUM
KW  - GENE EXPRESSION
KW  - GOLGI COMPLEX
KW  - IN VIVO STUDY
KW  - NONHUMAN
N2  - Expression and tracking of fluorescent fusion proteins has revolutionized our understanding of basic concepts in cell biology. The protocol presented here has underpinned much of the in vivo results highlighting the dynamic nature of the plant secretory pathway. Transient transformation of tobacco leaf epidermal cells is a relatively fast technique to assess expression of genes of interest. These cells can be used to generate stable plant lines using a more time-consuming, cell culture technique. Transient expression takes from 2 to 4 days whereas stable lines are generated after approximately 2 to 4 months
UR  - https://drive.google.com/file/d/188Y6FaZg15XWBOd6D3uSIM0dzPvh8dYS/view?usp=drivesdk
ER  -