01597nam a2200301Ia 4500 MX-MdCICY 20260521091541.0 CICY B-16905 Rapid, transient expression of fluorescent fusion proteins in tobacco plants and generation of stably transformed plants Nature Protocols, 1(4), p.2019-2025, 2006 Expression and tracking of fluorescent fusion proteins has revolutionized our understanding of basic concepts in cell biology. The protocol presented here has underpinned much of the in vivo results highlighting the dynamic nature of the plant secretory pathway. Transient transformation of tobacco leaf epidermal cells is a relatively fast technique to assess expression of genes of interest. These cells can be used to generate stable plant lines using a more time-consuming, cell culture technique. Transient expression takes from 2 to 4 days whereas stable lines are generated after approximately 2 to 4 months. ARTICLECELL FUSIÓN CELL TRANSFORMATION CULTURE TECHNIQUE ENDOPLASMIC RETICULUM GENE EXPRESSION GOLGI COMPLEX IN VIVO STUDY NONHUMAN Sparkes, I.A. Runions, J. Kearns, A. Hawes, C. https://drive.google.com/file/d/188Y6FaZg15XWBOd6D3uSIM0dzPvh8dYS/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 27009 27009 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-16905 2025-06-25 16:02:00 2025-06-25 REF1