Keles, D. Pinar, H. Ata, A. Taskin, H. Yidiz, S. Buyukalaca, S. text xx 9999 monographic und

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The most successful technique used to obtain haploid plant in pepper is anther culture. The chromosome content of haploid plants can be doubled spontaneously or using colchicine. In this study,we compared the rate of spontaneous doubled haploidy of different pepper types. Seven charleston, six bell, eight capia, and seven green pepper genotypeswere used as plant material. Murashige and Skoog (MS)nutrient medium with 4 mg·L-1 naphthaleneacetic acid (NAA), 0.5 mg·L-1 6-benzylaminopurine (BAP), 0.25 per cent activated charcoal, 30 g·L-1 sucrose, and 15mg·L-1 silver nitrate (AgNO3)was used. Ploidy levels of plants obtained through anther culture were detected using both flowcytometry and simple sequence repeats (SSR)markers. The results showed that different spontaneous doubled haploidy rates were obtained fromdifferent pepper types. The highest rate was observed in bell pepper type with 53.4 per cent (mean of six genotypes)of haploid plants undergoing spontaneouschromosome doubling. This was followed by charleston and capia types with 31.9 per cent and 30.4 per cent doubling, respectively. Green pepper type gave the lowest spontaneous doubled haploidy rate with 22.2 per cent doubling. The results obtained from this study will be useful both for future work on haploidy in pepper and for breeding programs. ANTHER CULTURE CAPSICUM ANNUUM PEPPER SPONTANEOUS CHROMOSOME DOUBLING https://drive.google.com/file/d/1B3n8BdojJhVgjw0D8IMWVcm4mzEbgGtX/view?usp=drivesdk https://drive.google.com/file/d/1B3n8BdojJhVgjw0D8IMWVcm4mzEbgGtX/view?usp=drivesdk 250602 20260521091536.0