02134nam a2200361Ia 4500003001000000005001700010040000900027245013700036490005700173520099900230650001001229650003501239650001501274650002801289650001201317650001501329650000701344650001901351650001701370650000701387650001201394650002701406650002401433650002201457650000801479650000801487650002601495650001901521700001901540700001601559856015601575008004101731MX-MdCICY20260521091533.0  cCICY10aGrowth, Cytology and Flow Cytometry of Embryogenic Cell Suspension Cultures of Panicum maximum Jacq. and Pennisetum purpureum Schum.0 vJournal of Plant Physiology, 123(3), p.211-227, 19863 aEmbryogenic cell suspensions of Panicum maximum (4 year old)and Pennisetum purpureum (3 year old)continue rapid growth upon subculture without a marked lag phase. They have cell doubling times of 27 h and 32 h, respectively, during the exponential phase of growth. Maximum cell numbers of 2 × 10? cells · ml-1 are reached on about the eighth day of subculture in both species. Thereafter, cell lysis and death occur progressively. By day 16, 90 percent of P. purpureum cells appear non-viable. Flow cytometry of the cell suspensions showed that at any given time during the 8-9 day culture period, a majority of the cells are in G1 phase of the cell cycle, with substantial numbers cycling through the S and G2 phases. No marked change in this distribution pattern was seen in the first 8-9 days of growth during which the cultures did not reach stationary phase. Flow cytometry, microspectrophotometry and chromosome counts showed the cultures comprised predominantly ofnormal diploid cells.14a2,4-D14a2,4-DICHLOROPHENOXYACETIC ACID14aCELL CYCLE14aCELL SUSPENSION CULTURE14aCEREALS14aDRY WEIGHT14aDW14aFLOW CYTOMETRY14aFRESH WEIGHT14aFW14aGRASSES14aMICROSPECTROPHOTOMETRY14aPACKED CELL VOLUMEN14aPANICUM MÁXIMUM14aPCV14aPCV14aSOMATIC EMBRYOGENESIS14aTISSUE CULTURE12aKarlsson, S.B.12aVasil, I.K.40uhttps://drive.google.com/file/d/1XsHxI796IuBZUMdFkeRZk8l94sdZbd-s/view?usp=drivesdkzPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx250602s9999    xx |||||s2   |||| ||und|d