01613nam a2200205Ia 4500 MX-MdCICY 20260521091530.0 CICY B-16570 Somatic embryogenesis of avocado (Persea americana)and its application for plant improvement Acta Horticulturae, 575, p.133-138, 2002 The avocado is cultivated in tropical and subtropical regions of more than 50 countries. Avocado improvement through conventional breeding is slow, especially for resistance to the devastating soil-borne disease Phytophthora root-rot (PRR). Biotechnology can supplement avocado breeding by generating new variability unobtainable using conventional approaches. Therefore, an improved protocol for plant regeneration by somatic embryogenesis has been developed. Culture initiation, maintenance and somatic embryo development have been optimized. Plant regeneration from protoplasts has also been described and somatic hybridization with sexually and asexually incompatible related PRR resistant Persea spp. such as P. cinerascens and P. pachypoda is in progress. Genetic transformation of embryogenic avocado cultures with an anti-fungal protein gene, chitinase and glucanase genes are underway. GENETIC TRANSFORMATION PROTOPLAST TISSUE CULTURE Witjaksono, Litz, R.E.E. https://drive.google.com/file/d/1EKre0VS6JNlZlxLeTT6bQsip2aHwWPe_/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 26679 26679 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-16570 2025-06-25 16:01:54 2025-06-25 REF1