01534nam a2200169Ia 4500003001000000005001700010040000900027245009700036490004500133520089900178650002701077650001501104650001901119700002901138856015601167008004101323MX-MdCICY20260521091530.0  cCICY10aSomatic embryogenesis of avocado (Persea americana)and its application for plant improvement0 vActa Horticulturae, 575, p.133-138, 20023 aThe avocado is cultivated in tropical and subtropical regions of more than 50 countries. Avocado improvement through conventional breeding is slow, especially for resistance to the devastating soil-borne disease Phytophthora root-rot (PRR). Biotechnology can supplement avocado breeding by generating new variability unobtainable using conventional approaches. Therefore, an improved protocol for plant regeneration by somatic embryogenesis has been developed. Culture initiation, maintenance and somatic embryo development have been optimized. Plant regeneration from protoplasts has also been described and somatic hybridization with sexually and asexually incompatible related PRR resistant Persea spp. such as P. cinerascens and P. pachypoda is in progress. Genetic transformation of embryogenic avocado cultures with an anti-fungal protein gene, chitinase and glucanase genes are underway.14aGENETIC TRANSFORMATION14aPROTOPLAST14aTISSUE CULTURE12aWitjaksono, Litz, R.E.E.40uhttps://drive.google.com/file/d/1EKre0VS6JNlZlxLeTT6bQsip2aHwWPe_/view?usp=drivesdkzPara ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx250602s9999    xx |||||s2   |||| ||und|d