02011nam a2200361Ia 4500 MX-MdCICY 20260521091523.0 CICY B-16383 Naturally occurring off-switches for CRISPR-Cas9 Cell, 167(7), p.1829-1838, 2016 CRISPR-Cas9 technology would be enhanced by the ability to inhibit Cas9 function spatially, temporally, or conditionally. Previously, we discovered small proteins encoded by bacteriophages that inhibit the CRISPR-Cas systems of their host bacteria. These "anti-CRISPRs" were specific to type I CRISPR-Cas systems that do not employ the Cas9 protein. We posited that nature would also yield Cas9 inhibitors in response to the evolutionary arms race between bacteriophages and their hosts. Here, we report the discovery of three distinct families of anti-CRISPRs that specifically inhibit the CRISPR-Cas9 system of Neisseria meningitidis. We show that these proteins bind directly to N. meningitidis Cas9 (NmeCas9)and can be used as potent inhibitors of genome editing by this system in human cells. These anti-CRISPR proteins now enable "off-switches" for CRISPR-Cas9 activity and provide a genetically encodable means to inhibit CRISPR-Cas9 genome editing in eukaryotes. Video Abstract © 2016 Elsevier ANTI-CRISPR CAS9 CRISPR-CAS GENOME EDITING NEISSERIA MENINGITIDIS PHAGE Pawluk, A. Amrani, N. Zhang, Y. Garcia, B. Hidalgo-Reyes, Y. Lee, J. Edraki, A. Edraki, A. Sontheimer, E.J. Maxwell, K.L. Davidson, A.R. https://drive.google.com/file/d/1Yak_VkTUJRGaA86vR2NMZKw53fbnCkFS/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 26496 26496 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-16383 2025-06-25 16:01:50 2025-06-25 REF1