Arcioni, S. Davey, M. R. Dos Santos, A. V. P. Cocking, E. C. text xx 9999 monographic und

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Mesophyll and cell suspension protoplasts of Medicago coerulea and M glutinosa divided to form callus. The conditions of plant growth, plant age, protoplast isolation, and protoplast cul-ture were critical for sustained division of mesophyll protoplasts. Those for division of cell suspension protoplasts were less stringent. 2,4-D in combination with a cytokinin induced embryogenesis in both Medicago species. In M. coerulea, BAP was effective as the cytokinin, while M. glutinosa had a requirement for zeatin. The potential of protoplast systems for transfer of characteristics from M. coerulea to M. glutinosa and M. sativa is discussed. MEDICAGO COERULEA M GLUTINOSA BAP CELL SUSPENSION PROTOPLASTS MESOPHYLL PROTOPLASTS DIVISION 2,4·D FORAGE LEGUMES GENETIC MANIPULATION SOMATIC EMBRYOGENESIS ZEATIN https://drive.google.com/file/d/17ZMoqWWZU0fW8IHL59tpreVejysmvb2n/view?usp=drivesdk https://drive.google.com/file/d/17ZMoqWWZU0fW8IHL59tpreVejysmvb2n/view?usp=drivesdk 250602 20260521091456.0