01714nam a2200337Ia 4500 MX-MdCICY 20260521091456.0 CICY B-15532 Somatic Embryogenesis in Tissues From Mesophyll and Cell Suspension Protoplasts of Medicago coerulea and M. glutinosa Z. Pjlanzenphysiol., 106, p.105-110, 1982 Mesophyll and cell suspension protoplasts of Medicago coerulea and M glutinosa divided to form callus. The conditions of plant growth, plant age, protoplast isolation, and protoplast cul-ture were critical for sustained division of mesophyll protoplasts. Those for division of cell suspension protoplasts were less stringent. 2,4-D in combination with a cytokinin induced embryogenesis in both Medicago species. In M. coerulea, BAP was effective as the cytokinin, while M. glutinosa had a requirement for zeatin. The potential of protoplast systems for transfer of characteristics from M. coerulea to M. glutinosa and M. sativa is discussed. MEDICAGO COERULEA M GLUTINOSA BAP CELL SUSPENSION PROTOPLASTS MESOPHYLL PROTOPLASTS DIVISION 2,4·D FORAGE LEGUMES GENETIC MANIPULATION SOMATIC EMBRYOGENESIS ZEATIN Arcioni, S. Davey, M. R. Dos Santos, A. V. P. Cocking, E. C. https://drive.google.com/file/d/17ZMoqWWZU0fW8IHL59tpreVejysmvb2n/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 25651 25651 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-15532 2025-06-25 16:01:34 2025-06-25 REF1