Somatic Embryogenesis in Tissues From Mesophyll and Cell Suspension Protoplasts of Medicago coerulea and M. glutinosa 

 -  Z. Pjlanzenphysiol., 106, p.105-110, 1982 .
<br/><br/> Mesophyll and cell suspension protoplasts of Medicago coerulea and M glutinosa divided to form callus. The conditions of plant growth, plant age, protoplast isolation, and protoplast cul-ture were critical for sustained division of mesophyll protoplasts. Those for division of cell suspension protoplasts were less stringent. 2,4-D in combination with a cytokinin induced embryogenesis in both Medicago species. In M. coerulea, BAP was effective as the cytokinin, while M. glutinosa had a requirement for zeatin. The potential of protoplast systems for transfer of characteristics from M. coerulea to M. glutinosa and M. sativa is discussed. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>MEDICAGO COERULEA<br/>M GLUTINOSA<br/>BAP<br/>CELL SUSPENSION PROTOPLASTS<br/>MESOPHYLL PROTOPLASTS<br/>DIVISION<br/>2,4·D<br/>FORAGE LEGUMES<br/>GENETIC MANIPULATION<br/>SOMATIC EMBRYOGENESIS<br/>ZEATIN