TY  - BOOK
AU  - Parry,M.A.J.
AU  - Godfrey Schmidt,C.N.
AU  - Cornelius,M.J.
AU  - Keys,A.J.
AU  - Millard,B.N.
AU  - Gutteridge,S.
TI  - Stimulation of Ribulose Bisphosphate Carboxylase Activity by Inorganic Orthophosphate without an Increase in Bound Activating CO2: Co-operativity between the Subunits of the Enzyme
KW  - RIBULOSE BISPHOSPHATE CARBOXYLASE
KW  - ACTIVATION
KW  - INORGANIC ORTHOPHOSPHATE
KW  - CO2
KW  - CO-OPERATIVITY
N2  - Ribulose bisphosphate carboxylase/oxygenase (E.C. 4.1.1.39)from wheat (Triticum aestivum L.), already activated by reaction with CO2 and Mg2+, was increased in activity on addition of inorganic orthophosphate. This further activation took place without a significant increase in the amount of bound activating CO2 and the effect was relatively greater with smaller amounts of bound CO2. With less than 2-0 mol of CO2 bound per mol holoenzyme, phosphate increased activity about five-fold whilst with 70 mol of bound activating CO2 per mol holoenzyme, phosphate increased activity by a factor of only 1 -8. This decrease in the effect of orthophosphate with increase in bound activating CO2 suggests negative co-operativity between activated sites. The stimulation of activity by inorganic orthophosphate must be a process distinct from activation by CO2; it was observed with both the slow and the rapidly activating forms of ribulose bisphosphate carboxylase/oxygenase from wheat
UR  - https://drive.google.com/file/d/1i_HuWPZYLc2SPAu0tU6YRNj9Bn3jKZav/view?usp=drivesdk
ER  -