01699nam a2200337Ia 4500 MX-MdCICY 20260521091428.0 CICY B-14660 Three Methods for the Introduction of Foreign DNA intoAgrobacterium Methods in Molecular Biology, 343, p.43-53, 2009 The genetic manipulation of Agrobacterium tumefaciensis used to facilitate studies of bacterial gene functions or as a first step in introducing genetic material into transformable plant cells through the use of T-DNA binary vectors. Three methods are commonly used. Transformation with purified plasmid can be done with either electroporation or a simple freeze/thaw transformation method. Alternatively, a mobilizable plasmid can be placed into Agrobacteriumusing the triparental mating method. Here we present three detailed protocols for Agrobacteriumstrain construction using electroporation, the freeze/thaw method of transformation, and triparental mating. ELECTROPORATION FREEZE/THAW TRIPARENTAL MATING CONJUGATION INCOM-PATIBILITY GROUP SELECTIVE PLATES COUNTERSELECTION DONOR RECIPIENT TRANSCONJUGANT SELF-TRANSMISSIBLE PLASMID ORIGIN OF TRANSFER (ORIT). Wise, A.A. Liu, Z. Binns, A.N. https://drive.google.com/file/d/19roaQ8d2vcliIUkWpFdlISlqf3bhTh0A/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 24785 24785 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-14660 2025-06-25 15:39:45 2025-06-25 REF1