02277nam a2200265Ia 4500 MX-MdCICY 20260521091349.0 CICY B-13422 Metabolite profiling reveals clear metabolic changes during somatic embryo development of Norway spruce (Picea abies) Tree Physiology, 32(2), p.232-244, 2012 Progress on industrial-scale propagation of conifers by somatic embryogenesis has been hampered by the differences in developmental capabilities between cell lines, which are limiting the capture of genetic gains from breeding programs. In this study, we investigated the metabolic events occurring during somatic embryo development in Norway spruce to establish a better understanding of the fundamental metabolic events required for somatic embryo development. Three embryogenic cell lines of Norway spruce (Picea abies (L.)Karst)with different developmental capabilities were studied during somatic embryo development from proliferation of proembryogenic masses to mature somatic embryos. The three different cell lines displayed normal, aberrant and blocked somatic embryo development. Metabolite profiles from four development stages in each of the cell lines were obtained using combined gas chromatography.mass spectrometry. Multivariate discriminant analyses of the metabolic data revealed significant metabolites (P . 0.05)for each development stage and transition. The results suggest that endogenous auxin and sugar signaling affects initial stages of somatic embryo development. Furthermore, the results highlight the importance of a timed stress response and the presence of stimulatory metabolites during late stages of embryo development. GAS CHROMATOGRAPHY-MASS SPECTROMETRY METABOLITE PROFILING METABOLOMICS NORWAY SPRUCE (PICEA ABIES) SOMATIC EMBRYOGENESIS Businge, E. Brackmann, K. Moritz, T. Egertsdotter, U. https://drive.google.com/file/d/1l9TQwbWOID01AZqSoVNygSXHJunTknqc/view?usp=drivesdk Para ver el documento ingresa a Google con tu cuenta: @cicy.edu.mx Loc REF1 250602s9999 xx |||||s2 |||| ||und|d 23561 23561 0 0 Loc 0 0 F1 CICY CICY RE 2025-06-25 0 B-13422 2025-06-25 15:39:22 2025-06-25 REF1